Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose
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چکیده
منابع مشابه
Preparation of D-Psicose from D-Fructose by Immobilized D-Tagatose 3-Epimerase
D-Tagatose 3-epimerase (D-TE) from Pseudomonas sp. ST-24 immobilized on Chltopearl beads of BCW 2503 was used in the preparation of D-psicose from D-fructose. When D-fructose solution (10%) was passed through a column packed with immobilized D-TE about 20% of the D-fructose was converted to D-psicose. This column was continuously used for 10 d at 4PC and about 90 g of D-psicose were obtained fr...
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Hygromycin A is interesting from the standpoint of the previously undescribed sugar it contains, 5-keto-6-deoxy-n-arabohexose (2,3). The pathway of biosynthesis of this compound is unknown. In this study, Streptomyces hygroscopicus was grown in the presence of specifically labeled glucose, and the distribution of radioactivity in the 5-keto-6-deoxy-n-arabohexose of hygromycin A was determined. ...
متن کاملPreparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate.
For easy measurement of 5-keto D-gluconate (5KGA) and 2-keto D-gluconate (2KGA), two enzymes, 5KGA reductase (5KGR) and 2KGA reductase (2KGR) are useful. The gene for 5KGR has been reported, and a corresponding gene was found in the genome of Gluconobacter oxydans 621H and was identified as GOX2187. On the other hand, the gene for 2KGR was identified in this study as GOX0417 from the N-terminal...
متن کاملD-fructose dehydrogenase of Gluconobacter industrius: purification, characterization, and application to enzymatic microdetermination of D-fructose.
D-Fructose dehydrogenase was solubilized and purified from the membrane fraction of glycerol-grown Gluconobacter industrius IFO 3260 by a procedure involving solubilization of the enzyme with Triton X-100 and subsequent fractionation on diethylaminoethyl-cellulose and hydroxylapatite columns. The purified enzyme was tightly bound to a c-type cytochrome and another peptide existing as a dehydrog...
متن کامل2-Keto-D-gluconate-yielding membrane-bound D-glucose dehydrogenase from Arthrobacter globiformis C224: purification and characterization.
Glucose dehydrogenase (GlcDH) is the rate-limiting catalyst for microbial conversion of glucose to the important organic acid 2-ketogluconic acid (2KGlcA). In this study, a D-glucose dehydrogenase was purified from the industrial 2KGlcA producer Arthrobacter globiformis C224. After four purification steps, the GlcDH was successfully purified over 180 folds and specific activity of 88.1 U/mg. A ...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2017
ISSN: 0916-8451,1347-6947
DOI: 10.1080/09168451.2016.1254535